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We investigated whether pre-forming acetylcholine receptor (AChR) clusters on engineered muscle fibers using an AChR cluster-inducing factor (agrin) prior to implantation would facilitate established contacts between implanted muscle tissues and nerves and result in rapid innervation of engineered muscle in vivo.
Lipid accumulation was detected in the TA muscle fibers using Oil Red-O staining following fixation in 3% paraformaldehyde according to Koopman et al. [18], nuclei were visualised by counterstaining with DAPI (Molecular Probes).
In addition to skeletal muscle homogenates we studied the expression levels separately in capillaries within the muscle and in pure muscle fibers using the LCM method.
Fiber counts were obtained by manually counting the number of muscle fibers using Image J from scanned H & E sections of the entire muscle (n = 5-6/group).
To do this, we performed simultaneous recordings of DDNs (using the loose patch configuration) and spinal motoneurons or muscle fibers (using the whole-cell configuration; n = 13).
Biometrics SX230 active (Ag/AgCl) electrodes separated by 2 cm were carefully taped to the belly of each muscle, parallel to the muscle fibers, using hypoallergenic adhesive tape and cotton wool swabs to minimize sweat induced interference.
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By learning to move your own body though space, you increase the amount of muscle fibers used.
The same protocol was used to confirm GFP expression in various types of muscle fibers, by using mouse anti-desmin antibodies (1 200, Dako UK, Ltd., Cambridgeshire, United Kingdom).
The same protocol for either in vitro myofibers or isolated muscle fibers was used.
In this study, we aimed to determine the nature and location of PTM sites using mass spectrometry in parallel with assessment of the catalytic and force-generating capacity of specific myosin isoforms using a modified single fiber in vitro motility assay and measure conformational changes during single muscle fiber contraction using small-angle X-ray scattering.
Modeling the muscle fiber direction using central line is possible, further validation is needed but the preliminary results showed here indicate a restoration of bipennate architecture in RF undergoing electrical stimulation treatment.
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