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Transgenic animals were monitored under fluorescent light during the larval and pupal stages to determine any muscle expression pattern.
All strains revealed the expected body muscle expression pattern (Additional file 1: Figure S5) with clear nuclear localization for the FPs equipped with a NLS (data not shown).
Muscle expression pattern were assessed using 74 Porcine Genome Array which contains 23,937 probe sets that interrogate approximately 23,256 transcripts from 20,201 S. scrofa genes.
Muscle expression pattern were assessed using 74 Porcine Genome Array which contains 24,123 probe-sets that interrogate 20,689 transcripts that were assigned to known genes [ 6]. Preparation of target products, hybridization and scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols using 5 μg of total RNA to prepare antisense biotinylated RNA.
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The scatterplot identifies genes with a muscle specific expression pattern, and whether the query sample matched that expression or not.
In addition, SRF is known to regulate its own muscle-specific co-activator MYOCD, which, together with SRF, forms a complex that is a master regulator of smooth muscle gene expression pattern [61].
A recent study profiling gene expression in the mammary gland side population also noted a muscle-specific expression pattern [60].
In wild-type hearts, Pol II occupancy at the Telokin promoter was equivalent to that of an untranscribed intergenic region, in agreement with Telokin's published smooth muscle-restricted expression pattern (Herring et al., 2001).
9– 12 For these reasons, we investigated skeletal muscle gene expression patterns in ALS patients to identify targets that might provide insight into disease-relevant pathways and identify biomarkers.
Furthermore, given the distinct roles of subcutaneous and intramuscular fat depots in cattle lipid metabolism, the tissue-specific variations were also investigated through the analysis of both SAT and muscle gene expression patterns.
Genes with a low tm-score (doesn't match muscle) and a negative ts-score (matches other tissues better) reside in the lower left corner of the plot, indicating genes with muscle specific expression patterns that do not match the query.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com