Smooth muscle contractility showed, at the first glance, no difference for the explants using different implant materials.
The explants used in the laboratory experiment were sourced from the reef flat at Miall Island.
The regeneration frequency was calculated through dividing the number of regenerated explants by total number of explants used.
The use of muscle explants in combination with defined matrix environments further demonstrated that distinct ECM components can differentially direct all of the cellular behaviors necessary for limb regeneration, including proliferation, migration, myofiber fragmentation and myoblast fusion.
Mature skeletal muscle relies on NOTCH1 for repair of injury,,, but NOTCH1 protein was previously not detected in myofibers of murine skeletal muscle explants although it was observed in the explants' satellite cells and Mb.
Myoblast cell populations were isolated from muscle biopsies using the explant culture technique [23].
The right frontal muscle, masseter muscle, temporal muscle, pterigoid muscle, sternocleidomastoid muscle, sternocleidomastoid muscle insertion, neck muscle insertion and trapezium muscle were examined using the TTS system.
The response of the explants to PLB formation varies from species to species and from explant to explant used (Teng et al. 1997).
We assessed 11β-HSD1 activity by measuring 11-DHC to corticosterone conversion in liver microsomes and muscle tissue explants and used HSD1KO mice as an additional control.
Explants dissected using this protocol were also used in the previous analysis of palate development in the Gad1 mutant explants [13].
