To detect SUMOylation in muscle cells, endogenous G9a was immunoprecipitated from proliferating C2C12 myoblasts transiently transfected with SUMO1.
The effect of overexpression of RIP140 in transgenic mice was examined focusing on the soleus muscle, where endogenous RIP140 levels are low and a putative repressive action may be most clearly observed.
Thus, cultured, developing or regenerating muscles are different from adult muscles, and endogenous genes frequently behave differently from promoter-reporter constructs.
Most dramatically, in immunofluorescence studies on the expression of TRPC isoforms in mouse skeletal-muscle fibres, endogenous TRPC1, TRPC4 and TRPC6 were clearly localized to the plasma membrane; however, TRPC3 in particular was found almost exclusively intracellularly in perinuclear structures reminiscent of the Golgi [ 21].
The β-gal mRNA was present in all the mouse muscle tissues since the β-gal gene is endogenous.
The possibility that an adaptive response to endogenous muscle antigens released upon muscle fibers degeneration, which may represent a common feature in acute injury as well as in IIM or MDs, would attribute to all of these conditions an auto-reactive component.
Usui S, Maejima Y, Pain J, Hong C, Cho J, Park JY, Zablocki D, Tian B, Glass DJ, Sadoshima J. Endogenous muscle atrophy F-box mediates pressure overload-induced cardiac hypertrophy through regulation of nuclear factor-kappaB.
In muscle constructs with endogenous ACh levels suppressed by the application of α-NETA, miniagrin increased AChR clustering and twitch force amplitude but failed to improve intracellular Ca(2+) handling and increase tetanus-to-twitch ratio.
Recent data have suggested that the genetic background of mdx mice affects endogenous muscle regeneration [64].
