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Inflammatory pathway activation and cytokine expression were analyzed in L6 muscle cells expressing myc-tagged GLUT4 (L6GLUT4 myc) exposed to 0.2 mM palmitate or palmitoleate.
Interestingly, a stationary population of human skeletal muscle cells expressing the CD34 surface protein have been found to differentiate in vitro into genuine brown adipocytes with a high level of UCP1 expression (Crisan et al., 2008), suggesting that human skeletal muscle cells have the potential to transdifferentiate into brown adipocytes.
We have consistently observed nuclear foci formation and MBNL colocalization in muscle cells expressing 200 CAG repeats (Fig. 8).
Labeling of F-actin with rhodamine phalloidin suggested that muscle cells expressing Bix1 have fewer, less organized myofibrils than do muscle cells in wild type embryos (Figure S5).
This is illustrated in Figure 4, which shows a merged brightfield and fluorescence reconstruction of pharyngeal muscle cells expressing GFP in the anterior part of the C. elegans body (a full 3D reconstruction is shown in Video S2).
In wild type embryos, muscle cells appear to be actively driving tail extension in cooperation with notochord cells, through their coordinate elongation, while in muscle cells expressing Bix1, muscle elongation is impaired, thus constraining tail extension [22].
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Western blotting analysis of a number of different types of cells indicated that cultured human vascular smooth muscle cells express Tie2 under the experimental conditions, although the level of expression is much lower than that in endothelial cells.
This is indeed the case; however, despite the fact that arterial smooth muscle cells express multiple isoenzymes of the GRK family (22), only GRK2 expression has been reported to be elevated in both hypertensive patients (8) and in rodent models of hypertension (9, 10).
Muscle cells express both PW1 and Hsp70, and we show that these two factors interact.
Muscle cells expressed the myosin heavy chain (MyHC) (Fig. 1E, F) and the muscle regulatory factor (MRF) MyoD (Fig. 1G, H) both in control and injured animals.
Ascidian muscle cells express homologs of vertebrate transcriptional regulators required for specification and differentiation of paraxial mesoderm, including Snail, Tbx6 and MyoD ([6]; reviewed in [7]).
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