Notch3 plays a key role in the functional and structural integrity of small arteries by regulating vascular smooth muscle cell differentiation, maturation and phenotypic behaviour (Artavanis-Tsakonas et al., 1999; Domenga et al., 2004; Wang et al., 2008).
This technique could be a cost-effective tool for in vitro skeletal muscle cell differentiation and maturation, with potential applications in tissue regeneration and drug development.
Our results show that these coatings promote committed muscle progenitor differentiation and stem cell maturation in vitro when compared to cells grown on traditional cell culture coatings.
Here we show that natural muscle-specific coatings can (i) be derived from decellularized, solubilized adult porcine muscle, (ii) contain a complex mixture of ECM components including polysaccharides, (iii) adsorb onto tissue culture plastic and (iv) promote cell maturation of committed muscle progenitor and stem cells.
Maturation includes smooth muscle cell recruitment, which appears to play an important role in the orientation of newly formed vessels [ 10].
The increases in transversal stiffness of the muscle cell membranes are connected to the development of the cytoskeleton, in particular at cell maturation.
Loss of oxidative defense and potential blockade of satellite cell maturation in the skeletal muscle of patients with cancer but not in the healthy elderly.
Endoglin Mediates Vascular Maturation by Promoting Vascular Smooth Muscle Cell Migration and Spreading.
Tian, H, Ketova, T, Hardy, D, Xu, X, Gao, X, Zijlstra, A, and Blobe, GC. "Endoglin Mediates Vascular Maturation by Promoting Vascular Smooth Muscle Cell Migration and Spreading". Arteriosclerosis, thrombosis, and vascular biology 37, no.
PDGFRβ dependence on NRP-1 walsolsignificantant, so NRP-1 must regulate PDGFRβ-dependent smooth muscle cell migration, proliferation and differentiation during vessel-wall maturation and repair.
