For expression in brain, heart, spleen and head kidney, the V2/3 values were <0.15, while in liver and muscle V2/3 values were more than 0.15 (Table 3).
The pairwise variation (Vn/n+1) analysis showed that except for muscle, V2/3 values in brain, heart, liver and head kidney were less than the proposed geNorm cut-off of 0.15, whereas in spleen, the required M value resulted only after the addition of fifth gene (V4/5 value) (Table 3).
Considering that normal muscle PO2 values vary from 15 to 30 mmHg, it is difficult to imagine that muscle hypoxia could explain SAHL in these patients.
No pigs in this study had muscle pH24h –values above 6.0 or pH35min below 5.8 indicating no cases of DFD or PSE meat.
In contrast, the initial muscle δ13C value (−18.6‰) was above the dietary value and increased to −17.5‰ by day 56 (Figure 1B).
The muscle δ15N value increased from 16.1‰ at day 0, reached its peak value (18.1‰) at day 42, and then decreased slightly to 17.8‰ at day 56 (Figure 1E).
In each case, parameters were selected to maximise image contrast between thermal lesions and un-sonicated regions, e.g. using a short echo time of 40 60 ms in the T2-weighted sequences, for an expected muscle T2 value of approximately 50 ms [22].
Since absolute P0 is dependent upon muscle size, P0 values were normalized for CSA [CSA = muscle mass/ L0 × 1.06; (Brooks & Faulkner, 1988) and were expressed as specific force (sP0; kN/m).
However, Mn did influence the content of abdominal fat (P<0.01), pH in breast muscle (P<0.05), b* value and MDA content in leg muscle (P<0.05).
Maximum shortening velocity (Vmax) of muscle has Q10 values of ∼1.5 (e.g. Coughlin et al., 1996).
