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The stereotyped vm2 muscle arm structure indicates a specific guidance mechanism regulating the muscle arm extension.
We show that the general vulval muscle morphology is not affected in lin-12 wy750) lin-12 wy750324) mutands, further supporting the notion that the phenotype of lin-12(wy750) is restricted on the mutantsarm structure.
We found that both the postsynaptic potassium channel UNC-103E and a neurotransmitter receptor SER-1B were enriched on vm2 muscle arms, further supporting that the muscle arm structure is functionally relevant postsynaptic specializations.
Furthermore, the single-copy insertion of the transgene UNC-103E::GFP potassium channel which specifically labels the vm2 muscle arms, as well as the artificial membrane marker mCD8::mCherry that delineates the entire outline of vulval muscles, was also completely absent in the muscle arm areas, suggestive of a complete loss of the muscle arm structure.
Similar(56)
Mapping and molecular identification of these mutations showed that they affected genes in LIN-12/Notch signaling pathway including lin-12/Notch, apx-1/DSL and sel-12/presinilin (Table 1 and Figure 1 figure supplement 3).> In lin-12 wy750) lin-12 wy750ls, while the mutant morphology of both vm1 animals whilelargely normal, the SER-1B::GFP labeled vmusclecle armorphologyes were cofpletely abotht.
To circumvent the occasional silencing of the UNC-40 transgene, we achieved UNC-40 expression in vm1 by single-copy insertion and observed the similar ectopic muscle arm-like structure.
Arrowheads indicate ectopic muscle arm-like structures.
Ectopic expression of UNC-40 in vm1 induces muscle arm-like structures.
The developmental age of muscle cells in vitro correlated with muscle arm development.
This developmental timing matches the relatively late window of vulval muscle specification and muscle arm formation.
The HSN presynaptic development occurs before vm2 muscle arm formation and is independent of vulval muscles.
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