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The degree of chimerism was determined by performing semi-quantitative genomic PCR of the WT allele of dystrophin using DNA samples from skeletal muscle, heart and tail (Fig. 1B for skeletal muscle and data not shown).
JS participated to experiments of electrotransfer and optical imaging of the muscle and data analysis.
However, the choice of the muscle and data analysis is not compelling.
Scores from the right and left sides of the body were combined for the same muscle and data from all raters were collected.
Then for each blot, the normalized signals were related to the total CaMKII signal for the respective resting (i.e., "nonstimulated") muscle and data pooled between experiments.
Similar(55)
Furthermore, with a larger sample size, data on common risk factors, testing of three different muscle groups and data on physical work capacity, we also address some of the limitations of earlier studies.
Of note, overexpression of IL-6 in a non-dystrophic animal model, although causes growth impairment, does not induce any active inflammation, necrosis, regeneration or fibrosis in muscle (34 and data not shown).
For most tissues, data from just 1-3 experiments was available, but for well-studied tissues (liver, muscle and heart), data was available from as many as 10 separate experiments (Table 1).
In fact, numerous muscles beds, including the soleus, the diaphragm and heart muscles appeared relatively normal in these animals, despite that they express almost equivalent levels of UCP1 protein than in severely affected muscles such as the gastrocnemius or the tibialis anterior muscles ([14] and data not shown).
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No transcripts were found in other organs, including barbels, lips, heart, gills, muscle and brain (data not shown).
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