Sentence examples for muscle Growth medium from inspiring English sources

Primary human myoblast cultures were isolated by protease digestion from fresh muscle biopsies (collagenase II, dispase1, trypsin/EDTA) and expanded in skeletal muscle growth medium including supplement mix (PromoCell, Heidelberg, Germany), 10% FCS, glutamine (3 mM) and gentamycin (40 µg/ml).

Myoblasts were cultured in Skeletal Muscle Growth Medium (PromoCell) supplemented with 10% foetal calf serum, 1.5% l-glutamine and 300 µl of gentamicin.

Muscle cells were grown in skeletal muscle growth medium (SGM; PromoCell, Heidelberg, Germany), supplemented with 4 mM l-glutamine and 10% foetal bovine serum (FBS).

Muscle cells were grown in skeletal muscle growth medium (PromoCell, Heidelberg, Germany), supplemented with 4 m m l-glutamine and 10% foetal bovine serum and cultured as recommended by the supplier.

Differentiation and fusion into multinucleated myotubes was induced at 70% confluence by replacing skeletal muscle growth medium with serum-reduced fusion medium (Dulbecco's modified Eagle's medium supplemented with 2% horse serum and 2 mM glutamine) for 6 days.

Muscle cells were grown in skeletal muscle growth medium (PromoCell, Heidelberg, Germany), supplemented with 4 mM l-glutamine and 10% foetal bovine serum and cultured as recommended by the supplier.

Commercially available CASMCs (purchased from Lonza, Walkersville, MD, USA) were grown in Smooth Muscle Growth Medium-2 (SmgM-2; Lonza).

Human smooth muscle cells (Lonza, Porrino, Spain) were cultured in SmGM-2 Smooth Muscle Growth Medium-2 (Clonetics), containing 5% FBS.

Normal human aortic smooth muscle cells (SMC) were cultured with smooth muscle growth medium-2 (smGM-2; Lonza, Switzerland) supplemented with smGM2 SingleQuots supplements and growth factors kit (Lonza, Switzerland).

Human skeletal myoblast cell line (obtained from Vincent Mouly, Institut de Myologie, Paris, France) was maintained in skeletal muscle cell growth medium using skeletal muscle cell growth medium kit (Promocell, Heidelberg, Germany) supplemented with 20% fetal calf serum and penicillin streptomycin (1% (v/v); all supplied by Life Technology, Carlsbad, CA, USA).

The skeletal muscle cells were maintained in Skeletal Muscle Cell Growth Medium (SkGM) supplemented by the SkGM BulletKit (Lonza).