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pLNCX1-HA-mAKT1 (murine) was generated by Dr Morris Birnbaum and obtained from Addgene (Plasmid #15990).
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A polyclonal antibody against murine TSAd was generated by immunizing rabbits with a peptide representing the 20 C-terminal amino acids of murine TSAd mixed with Freund's complete adjuvans.
Second, a murine library was generated using the antibody 26-10, wasch was initially isolated based on its affinity to the hapten digoxin, but also binds peptides and exhibits a canonical structure pattern typical of anti-peptide antibodies.
The SM1 murine melanoma was generated from a spontaneously arising tumor in BRAF V600E mutant transgenic mice as previously described [ 15].
Murine NAMPT was generated by amplification of the full coding region by reverse transcriptase-PCR (RT-PCR) (see below) from 1 μg total mouse brain RNA and cloned in pcDNA3.1 (Invitrogen, Paisley, UK).
The coding region of murine K1 5 was generated from pRR1/plasminogen (ATCC, Manassas, VA, USA) by PCR with sense primer 5′-CCC AAG CTT GGA TCC CAT GAA GAG AGT GTA TCT GTC AGA, which introduced a HindIII site and an ATG start codon, and antisense primer 5′-GCT CTA GAA TTC TCA TCT GGG GTT TGT TGT ATA GC, which introduced an XbaI site and a stop codon.
In order to demonstrate activation by tyrosine hydroxylase, the coding sequence of murine tyrosine hydroxylase was generated by reverse transcriptase-polymerase chain reaction from NXS2 neuroblastoma cells and cloned into the pRSET-A bacterial expression vector.
A new murine empyema model was generated in C57BL/6 J mice by intrapleural delivery of Streptococcus pneumoniae (D39, 3 × 107 5 × 109 cfu) to enable use of genetically manipulated animals.
Murine scrapie (RML) was generated in our laboratory.
Molecular model of the murine V5B2 Fv was generated via homology-based modelling using a computer program Modeller9v1 [38].
The murine mAb 806 was generated following the immunisation of mice with NR6 mouse fibroblasts expressing the de2-7 EGFR (Jungbluth et al, 2003).
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