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In this report, we discuss the application of a methodology for high-contrast, high-resolution magnetic resonance microscopy (MRM) of murine tissue using a 3.0-T imaging system.
This article details the validation of this antibody for immunohistochemistry of formalin-fixed paraffin embedded murine tissue using a Leica BondMax immunostainer.
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Total RNA was isolated from murine tissues using TRIzol Reagent (Invitrogen, Cat #15596-026), and mature mRNA was extracted from total RNA using the Poly(A Purist kit (Ambion, Cat #1916) as per the manufacturers' standard protocols.
Total RNA was isolated from murine lung tissue using Trizol Life Technologiess, Paisley, UK) and cDNA was generated using oligo-dT primers with the Superscript II RNase reverse transcription system (Invitrogen, Paisley, UK).
This course provides a basic foundation in identification of human and murine tissue types, using a lecture-laboratory format.
Total RNA was isolated from 70-80% confluent MDA-MB-231 cells and from human and murine tissue samples using the RNeasy mini kit (QIAGEN).
To assess the effects of IL-4 or/and IL-10 expression on treatment of TNBS-induced murine colitis, we intraperitoneally injected these plasmids into mice and then detected these transgenic expressions in murine colon tissues using qRT-PCR.
The differences of pinopode development between each group of murine tissue were analyzed using a chi-square test.
Overlaps of human genes from human tissue and converted genes from murine tissue were determined using a newly developed Excel Macro.
Therefore, we aimed to study the effect of pemetrexed dose and perfusion temperature on the resultant pemetrexed concentration in 3 different compartments (systemic circulation, portal circulation and peritoneal tissues) using a murine model.
Immunohistochemical studies for PrP were initially performed in the mid-1990s on IBNC brain tissues using antibodies raised to murine PrP.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com