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(ii) Plant miRNAs were detected not only in circulation, but also in all murine tissue types examined, and at copy numbers rivaling those of endogenous miRNAs.
[13] developed a phylogenetic conservation and motif based approach to predict tissue specific enhancers, which allowed them to annotate ∼5,500 high-confidence mouse tissue-specific enhancers for 61 murine tissue types by integrating tissue-specific expression data, conservation information, and cis-regulatory motifs.
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DNMT1 was found to be the sole detectable DNA methyltransferase in all murine tissues and cell types examined till date.
The application of a standardized and quantitative technique, TaqMan RT-PCR, should give information about the pattern and relative importance of TRP channels for murine tissues and cell types.
Looking at Lsd1 gene expression across numerous murine tissues and cell types using the BioGPS.org (Wu et al. 2009) online platform (Affymetrix probe sets for murine Lsd1: 1426761_andand 1426762_s_at), it seems that, with few exceptions, Lsd1 mRNA is expressed at similar levels throughout murine tissues/cell types.
Investigation of expression data from a broad range of murine tissues and cell types confirmed that upregulated TFs are expressed in many distinct and non-overlapping lineages (supplementary material Fig. S2), demonstrating that loss of Brg1 does not result in differentiation of ESCs towards a single, non-mesodermal lineage.
Using Western blot and IHC on murine wild-type and knockout tissue we have demonstrated that this antibody to ARID1A correctly stains murine tissue by immunohistochemistry. Following reviewers comments, the authors have modified the mansucript to reflect their views.
Murine tissue samples were obtained from NMRI strain.
The band at 26 28 kDa in murine tissue colocalizes with BAMBI overexpressed in 293T cells.
Paraffin-embedded tissue was sectioned at 5 µm for human tissue and 10 µm for murine tissue onto glass slides.
Antibodies were administered i.p. and murine tissue staining for bound antibodies performed 24 48 h later.
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