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Immunohistochemical studies were carried out with the biotinylated scFv173 on human normal and tumour samples, and on murine tissue sections.
Were such slides run on the murine tissue sections and can the results be included in the data.
Our first experimental data suggesting the formation of SG protein adducts was the detection of immunoreactive satratoxin in murine tissue sections and cells obtained by bronchoalveolar lavage.
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FFPE murine xenograft tissue sections were deparaffinized in xylene, and rehydrated in graded alcohols.
To investigate the potential association of neuroserpin and tPA with cerebral vessels we performed immunofluorescent staining on WT murine brain tissue sections and analyzed the expression of neuroserpin and tPA by confocal microscopy.
45 Immunofluorescent staining of WT murine brain tissue sections confirmed expression of PDGFR α in GFAP-positive perivascular astrocytes in the neurovascular unit (Fig. 6A) and showed that the receptor is distributed in the border between the astrocytes and the vascular mural cells, here visualized by ASMA (Fig. 6B).
For each murine gut tissue section, 5 μg total RNA was used as the starting material for all individual samples.
IHC staining of human (Online resource, Table 1) or murine frozen normal tissue sections did not show any positive staining except for weak staining of normal human breast.
In order to define the infiltrating inflammatory cells in the acute and chronic phases of murine MIRP, we stained tissue sections dissected from cartilage of nasal, laryngeal, and tracheal specimens.
To distinguish xenografted human tumor cells from murine cells we stained tissue sections with the human-specific STEM121 antibody (Fig. 5F G and S3A C, lower panels), and found that this staining overlapped well with the H&E staining (Figs. 5F, S3A C).
The tissue distribution of murine CD146 was first analyzed by immunohistochemistry using tissue sections of healthy murine organs.
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