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Murine tissue samples were obtained from NMRI strain.
EST counts from different human and murine tissue samples were extracted for 10 human and 8 mouse genes from the UNIGENE website [ 33].
Total RNA was isolated from 70-80% confluent MDA-MB-231 cells and from human and murine tissue samples using the RNeasy mini kit (QIAGEN).
To prepare protein extract, we homogenized murine tissue samples with T-PER (Pierce, Rockford, IL), using a 1: 20 ratio of tissue-to-sample preparation reagent, as directed by the manufacturer's instruction.
Expressed sequence tag (EST) profiles from several human and murine tissue samples were extracted for COG genes from the UniGene database [ 48] as EST counts per million transcripts and were log2 transformed (Homo sapiens: UniGene Build #223 and Mus musculus: UniGene Build #183).
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Fig. 1 S100A6 mRNA levels are up-regulated in human and murine CCA tissue samples.
In accordance with this finding, we showed that S100A6 is significantly overexpressed in human and murine CCA tissue samples, corroborating a pathophysiological role of S100A6 in cholangiocarcinoma.
Tunnel or PCNA-positive cells in murine synovial tissue samples were counted in a blinded manner in ten high-power fields per section, an average score was obtained and results expressed as the number of Tunnel or PCNA-positive cells mm2 using Histolab™ microvision version 5.9.2.
Whilst for the purposes of this study, the identification of each individual protein contributing to the final mathematical model was not necessary; we were interested to explore the possibility of identifying differentially expressed proteins from the murine brain tissue samples which may be important in disease mechanisms.
In this study, we investigated murine distal ileum tissue samples from two different age groups (3 and 18 months).
BbCRASP expression in cultured spirochetes grown in vitro to a density of 107/ml and in infected murine skin and heart tissue samples at 12 days of infection is presented (Fig. 6).
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