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Additionally, this post-mitotic function for cohesin appears not to be limited to neurons as immunoblots demonstrate that cohesin is differentially expressed in a variety of murine tissue extracts [32].
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NAMPT concentration was determined in murine sera and tissue extracts using a mouse visfatin/PBEF ELISA kit (Circulex, LabForce, Nunningen, Switzerland) NAMPT localization was studied by immunohistochemistry (IHC) using a rat monoclonal antibody against murine NAMPT[4] on paraffin-embedded sections of paws.
EST counts from different human and murine tissue samples were extracted for 10 human and 8 mouse genes from the UNIGENE website [ 33].
Expressed sequence tag (EST) profiles from several human and murine tissue samples were extracted for COG genes from the UniGene database [ 48] as EST counts per million transcripts and were log2 transformed (Homo sapiens: UniGene Build #223 and Mus musculus: UniGene Build #183).
We also found that tissue extracts from murine corneas inhibited T-cell proliferation, which could be partly reversed by either nor-NOHA or BEC or by supplementation of the medium with l-arginine, thus demonstrating the presence of functional arginase in the cornea.
To prepare protein extract, we homogenized murine tissue samples with T-PER (Pierce, Rockford, IL), using a 1: 20 ratio of tissue-to-sample preparation reagent, as directed by the manufacturer's instruction.
Tissue extracts were prepared.
Murine tissue samples were obtained from NMRI strain.
The band at 26 28 kDa in murine tissue colocalizes with BAMBI overexpressed in 293T cells.
Total quadriceps tissue extracts.
Paraffin-embedded tissue was sectioned at 5 µm for human tissue and 10 µm for murine tissue onto glass slides.
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