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All experiments were performed in a syngeneic murine system using either the mouse hepatoma cell line Hepa1-6 (derived from C57/LJ mice) or C56BL/6 mice.
However, further studies of the murine system, using 3D whole mount imaging of homeostatic tissue is required to establish whether these differences are genuine.
Zhou and coworkers convincingly displayed this IRAK-M transmitted NF-κB activity in the murine system using IRAK-1/IRAK-2 deficientficells cells and IRAK-1/IRAK-2/IRAK-M triple deficient cells derived from knockout mice [ 13].
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Given that no truly reliable preclinical animal model for human myeloma is available, we have used a murine xenograft model system using athymic NU/J Foxn1 nu mice transplanted intraperitoneally (i.p).
Extensive myelopoiesis is a well-described phenomenom in IFN-γR KO mice challenged with (auto antigen in complete Freund's adjuvant (CFA; reviewed in [ 16, 17]), so in an in vitro system using murine embryo fibroblast (MEF) cells we verified whether IL-17 may directly be involved in the induction of myelopoietic cytokines and/or chemokines and whether IFN-γ may influence this process.
To understand mechanisms regulating alternative splicing events important for cell death gene function, we previously established a model system using the murine caspase-2 (casp-2) gene.
They checked the biodistribution of this system using PET scan in the murine tumor model after intravenous injection.
The application of SD-ODT to coronary arteries may lead to a better understanding of vascular flow dynamics and the causes and treatment of CAD. Figure 1 shows a diagram of the SD-ODT system used to image murine epicardial and intra-myocardial coronary arteries.
The remaining differences between the fruit fly and murine models must be viewed in light of the system used.
The advantage of the LLC model is that implanted cells are immunologically compatible with the murine system, unlike the widely used xenograft models in which human cells are implanted into mouse tissue.
Compared to human TAP, murine TAP's binding specificity has not been characterized as well, even though murine systems are widely used for basic studies of antigen processing and presentation.
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