Sentence examples for murine somatic cells from inspiring English sources

Although noncancerous immortalized cell lines have been developed by introducing genes into human and murine somatic cells, such cell lines have not been available in large domesticated animals like pigs.

In order to determine whether viable iSCNT blastocysts can be developed for potential stem cell derivation, we have transferred murine somatic cells into enucleated porcine oocytes.

We then depleted porcine oocytes of their mtDNA using 10 µM 2',3'-dideoxycytidine and transferred murine somatic cells along with murine embryonic stem cell extract, which expressed key pluripotent genes associated with reprogramming and contained mitochondria, into these oocytes.

The groundbreaking work by Yamanaka in 2006 demonstrated that ectopic expression of defined transcription factors could reprogram murine somatic cells to iPS cells.

It is worth mentioning that murine somatic cells have longer telomeres compared to human cells, which significantly decreases the replicative senescence and increases the regenerative capacity of mdx satellite cells (compared to DMD satellite cells).

These homologs, particularly the Xenopus POUV proteins, can support ESC self-renewal in the absence of Oct4 [ 9, 12] and induce pluripotency in the reprogramming of human and murine somatic cells [ 5].

Consequently, as shown in Figure 4C, murine ESC extract containing immature mitochondria enhanced embryonic development in porcine oocytes and their development to blastocyst whilst murine somatic cell extract containing more mature mitochondria had an adverse effect on preimplantation development.

By injecting enucleated mtDNA-depleted porcine oocytes simultaneously with murine ESC extract and a murine somatic donor cell, we generated 207 reconstructions that produced 2-cell, morula and blastocyst stage embryos at rates of 63.288±1.16% (n = 131), 7.24±0.64% (n = 15) and 3.38±0.1% (n = 7), respectively (see Figure 4D).

Carey, B. W. et al. Reprogramming of murine and human somatic cells using a single polycistronic vector.

Recently the groundbreaking work demonstrated that ectopic expression of defined transcription factors (Oct4, Sox2, Klf4, c-Myc, Nanog, Lin28) could reprogram murine and human somatic cells to induced pluripotent stem cells (iPSCs).

It has been demonstrated that the level of cyclins and the activity of CDKs oscillates with a lesser amplitude in mESCs (murine ESCs) than in somatic cells due to a high level of expression of the APC/C (anaphase-promoting complex) inhibitor Emi1 [ 6].