Sentence examples for murine serum for from inspiring English sources

Briefly, 5 nmol of 111In-tilmanocept was incubated in 0.5 ml of murine serum for 90 min at 37 °C.

For wells that included 6-oxo-M1dG or structural analogues, the serum dilution used was 1 5000 in PBS-Tween (b), and the compounds depicted in Figure 1 were preincubated in the dilute murine serum for 45 min prior to the serum addition step.

Fig. 2 SDS-PAGE analysis of 111In-tilmanocept. 1 111In-tilmanocept sample, which was incubated in murine serum at 37 °C for 90 min. 2 111In-acetate, which was used as a low molecular weight radioactivity marker on the same gel.

A total of 70 ng/μl purified protein was incubated with 50% murine serum at 37 °C for different time periods between 0 and 48 h.

CC-10 TAg transgenic mice were injected in the axillary lymph node region with murine recombinant CCL19 (0.5  μg injection−1; Pepro Tech, Rocky Hill, NJ, USA) or normal saline diluent, which contained equivalent amounts of murine serum albumin (Sigma Chemical Co., St Louis, MO, USA) as an irrelevant protein for control injections.

All samples were blocked for unspecific binding by preincubation with 10% heat-inactivated murine serum (in-house production).

The optimal concentrations for in vitro angiostatin formation were investigated by incubating murine serum with uPA, tPA, and/or a free sulfhydryl group donor, followed by western blot analysis of the reaction products.

The latter route resulted in the highest sustained levels of human cytokines in the murine serum.

Sepp concentrations in murine serum were determined by Western blot as described recently [24].

To test stability the constructs were diluted 1∶10 with murine serum, cell culture media or ascites from mice with peritoneal carcinomatosis and incubated at 37°C for the indicated times.

After cooling, slides were blocked during 1 h with 1% murine serum (Sigma) diluted in Tris-buffer saline (TBS) (50 mmol/l Tris, 0.5 mol/l NaCl; pH 7.36) for preventing non-specific adsorption.