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Laminins (laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Sigma) was used at dilution 5 µg/ml for laminins overlay assay.
The protein concentration of the extracts was determined using a micro-BCA assay and equal amounts of protein were loaded for each sample and control laminin sample (Engelbreth-Holm-Swarm murine sarcoma, basement membrane) (37.5 µg).
We used laminin-111 (also known as laminin-1) from Engelbreth Holm Swarm murine sarcoma basement membrane to culture neoCMs in vitro.
Culture cell flasks (25 cm, Greiner Bio-One) were coated with a PBS solution containing 500 μg/ml Collagen I (rat tail, Marathon Lab), 40 μg/ml laminin (Engelbreth-Holm-Swarm murine sarcoma, basement membrane, Sigma), 50 μg/ml fibronectin (human plasma, Sigma) and 50 μg/ml phenol red (Sigma) as pH indicator.
The following materials were used as chemoattractants in separate assays: tissue culture medium with 10% FBS, medium conditioned by the two types of fibroblasts (NF and TF), fibronectin (from human plasma, Sigma, 25 μg/mL), and laminin-1 (from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Sigma) diluted in serum-free medium to 25 μg/mL.
Similar(55)
v-raf murine sarcoma viral oncogene homolog B. Computed tomography.
Murine sarcoma S180 cell line was obtained from SLAC Laboratory and reproduced in our laboratory.
VRAF murine sarcoma viral oncogene homologue B1 (BRAF) kinase has been considered to be a promising therapeutic target for various human cancers.
PURPOSE: About 65%to70%0% of melanomas harbor a mutation in v-raf murine sarcoma viral oncogene homolog B1 (BRAF) that causes the steady-state activation of extracellular signal-regulated kinase (ERK).
Engelbreth-Holm-Swarm mouse sarcoma basement membrane matrix (3D BM-matrix) was purchased from BD Biosciences.
The MOS (V-mos Moloney murine sarcoma viral oncogene homolog) gene has been previously identified as hypermethylated in ALL but not in AML [17].
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