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Human BM cells were cultured following the murine protocol to generate lowGM-DC.
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Colony forming cell assays involved the murine protocol from Stem Cell Technologies.
Here we described a method to generate human immature and maturation-resistant DC from BM precursor cells which was adopted from a murine protocol [23].
Our laboratory uses a modification of the CBD protocol when isolating rodent islets (see Appendix A for a annotated murine protocol and Appendix B for an abbreviated version).
Here, we establish a highly efficient protocol to freeze murine BM cells and further generate BMDM.
To address this question we used an in vitro differentiation protocol to induce murine ES cells to form spinal cord motor neurons (MN), which control muscle movement.
The A2UCOE-, SFFV-, or CMV-EGFP lentiviral vectors were used in an ex vivo protocol to transduce C57BL/6J murine bone marrow derived HSCs that were then engrafted into lethally irradiated recipients.
This report also details a flow cytometry-based protocol to measure the production of murine IFNα/β by splenic pDC.
Thus, we established and validated a protocol to obtain extra- and intracellular MPO from murine organs.
First, we utilized a double-staining protocol to determine the number of macrophages that had engulfed neutrophils in the murine BM under steady state.
We present here a protocol to visualize in vivo the behavior of Plasmodium berghei parasites in the hepatic tissue of the murine host.
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