Sentence examples for murine preosteoblastic cell from inspiring English sources
To evaluate the effects of ionizing radiation (IR) on murine preosteoblastic cell differentiation, we directed OCT-1 cells to the osteoblastic lineage by treatment with a combination of β-glycerophosphate (β-GP), ascorbic acid (AA), and dexamethasone (Dex).
Moreover, antagonism of endogenous BMP signalling reduces the osteogenic differentiation of a murine preosteoblastic cell line [ 10].
The partially differentiated murine preosteoblastic cell line, MC3T3-E1 cells (ATCC, USA) were cultured in MEM alpha (Life Technologies, Grand Island, NY, USA) supplemented with 10% heat-inactivated FBS, 100 U/ml penicillin, 100 g/ml streptomycin and 1% L-glutamine.
A murine preosteoblastic cell line, MC3T3-E1 (subclone 4, American Type Culture Collection, Manassas, VA), was cultured with growth media composed of alpha minimal essential medium (α MEM) (Mediatech, Manassas, VA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (PS) in a 5% CO2 incubator at 37°C.
Similar behaviors have also been observed in MMP-sensitive PEG hydrogels, where murine preosteoblastic cells (MC3T3-E1) were able to spread and migrate faster in more compliant hydrogels.
The murine multipotent mesenchymal progenitor cell line C2C12 and the preosteoblastic cell line MC3T3-E1 were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).
In contrast, strontium ranelate was shown to enhance preosteoblastic cell replication and collagen synthesis in culture without affecting bone mineralization.
This approach was investigated using the example of preosteoblastic cell response in vitro to fibroblast growth factor-2 (FGF-2) printed on fibrin films.
To verify the effect of RA on osteoblast differentiation, AJ18 expression level was examined using a rat clonal preosteoblastic cell line, ROB-C20 (C20).
Similarly, in mice and rat cell lines Sp7/osterix expression was found in the preosteoblastic cell line, MC3T3-E1, but not in fibroblasts, nor plasmacytoma or pheochromocytoma cells [ 5].
This dose dependent effect of alendronate was also supported by previous studies showing that bisphosphonates increase bone marrow-derived preosteoblastic cell proliferation and inhibit the apoptosis of osteocytes and osteoblasts at low concentrations [ 3, 21].
