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By applying the Phage Display technique to murine neural stem cells we isolated a peptide, KLPGWSG, present in proteins involved in both stem cell maintenance and differentiation.
This study aimed to investigate how different surface coatings affect the cytotoxicity and cellular uptake of silver nanoparticles (AgNPs) in murine neural stem cells (mNSCs).
Hence, we synthesized two LDLK-12-based self-assembling peptides functionalized with KLPGWSG peptide (KLP and Ac-KLP) and characterized them via atomic force microscopy, rheometry and circular dichroism, obtaining nanostructured hydrogels supporting murine neural stem cells differentiation in vitro.
We report a tissue engineering technique to print murine neural stem cells (C17.2), collagen hydrogel, and GF (vascular endothelial growth factor: VEGF -releasing fibrin gel to construct an artificial neural tissue.
Because of these limitations, most studies have focused on evaluating murine neural stem cells.
More recently, murine liver, stomach [8], lymphocyte, β[13], and murine neural stem cells (NSCs) [14] [16] were also capable of iPs induction.
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The in vitro murine neural embryonic stem cell test (ESTn) was designed as an alternative for neurodevelopmental toxicity testing.
We show that KLPGWSG binds molecules expressed on the cell surface of murine adult neural stem cells, thus may potentially be involved in stem cell fate determination.
These highlighted cell cycle components as a major proportion of differentially expressed genes that were directly down-regulated during differentiation of murine NSCs (neural stem cells) [ 60].
Here we show that functionalized α-helical-peptide hydrogels can be used to induce attachment, migration, proliferation and differentiation of murine embryonic neural stem cells (NSCs).
To test this, we generated data comparing expression between twelve single, murine embryonic neural stem cells in a series of pairwise hybridizations that were repeated as dye-swapped replicates.
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