Sentence examples for murine lungs using from inspiring English sources

Since glycosaminoglycan side chains of proteoglycans act as chemokine co-receptors in inflammation, we have used a CXCL8-based dominant-negative mutant, dnCXCL8, to displace neutrophil-related chemokines in murine lungs using models of lung inflammation.

Total RNA was purified from murine lungs using the RNeasy Mini Kit (Qiagen) with DNase treatment.

Total RNA was isolated from snap-frozen, murine lungs using the Nucleo Spin RNA/Protein isolation Kit (Macherey Nagel, Düren, Germany), quality checked and quantified using the NanoDrop ND-100 Spectrophotometer (Peqlab, Wilmington, USA).

Kretschmer, S. et al. Visualization of intrapulmonary lymph vessels in healthy and inflamed murine lung using CD90/Thy-1 as a marker.

We therefore identified miRNAs that are differentially regulated in a time-dependent manner during the early and later recovery phases of the lungs using a murine influenza infection model.

Total RNA was isolated from murine lung tissue using Trizol Life Technologiess, Paisley, UK) and cDNA was generated using oligo-dT primers with the Superscript II RNase reverse transcription system (Invitrogen, Paisley, UK).

However, despite this hypothesis on DC function, a recent study by Winter et al (2007) showed that increasing the murine lung myeloid DCs using FMS-like tyrosine kinase 3 ligand (Flt3L) negatively affected clearance of pneumococci and increased the mortality.

The expression of ccl-17, tslp,  and hprt-1 (internal control) genes in murine lungs was analyzed by qPCR using SYBR green-based master mix and RNA-specific QuantiTect primer assays (QIAGEN, Valencia, CA, USA) for mouse.

Mouse sIL-18Rβ (genebank accession number AK053176) was cloned from murine lung cDNA and used for the generation of an adenoviral vector (Ad.5IL-18Rβ).

To elucidate the mechanisms underlying this relationship, we studied the effects of Txnip overexpression on HIF-mediated transcription using murine lung epithelial (MLE-12) cells.

Further studies investigating the presence and distribution of metastases in vivo using murine lung cancer xenograft models are needed.