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As a control for the double knockouts and to determine whether p53 plays any role in normal murine lens development, we generated Trp53 CKO lenses.
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Overall, this study has allowed us to revisit some of the mechanisms involved in early lens development, has provided us with insights into the fate of cells during this rapid phase of murine lens growth, and has provided a novel method to study the rate of new fiber cell differentiation over a defined period of lens development and growth.
During development of the murine lens, fibre cells terminally differentiate – laying down a complex crystalline substructure that is devoid of nuclei and organelles, thus preventing light scattering and allowing normal lens transparency (Tholozan and Quinlan, 2007).
The involvement of homologous proteins in vertebrate lens development was subsequently elucidated by the characterization of mutations that cause congenital human or murine ocular disorders and their comparison to mutations in model organisms [1].
Growth factor regulation of lens development.
Rbm5 has a distinct expression pattern during lens development.
Cvekl, A. & Duncan, M. K. Genetic and epigenetic mechanisms of gene regulation during lens development.
Cvekl, A. & Ashery-Padan, R. The cellular and molecular mechanisms of vertebrate lens development.
Cavalheiro, G. R. et al. N-myc regulates growth and fiber cell differentiation in lens development.
This paper briefly examines the Fresnel lens development since 1970s and investigates the losses inherent in the linear Fresnel lenses.
This interaction supports the dual roles of filensin in the lens; roles that could be important during lens development.
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