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The study by Rinn et al. identified 27 sexually dimorphic genes in murine kidneys with at least >3 fold (p<0.001) change [20].
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In brief, murine kidneys were perfused with sterile culture medium (Ham's F12; GIBCO) containing 1 mg/ml collagenase (type II; Sigma) and 1 mg/ml pronase E (type XXV, Sigma) at pH 7.4 and 37°C.
Both human and murine kidneys showed bands reacting with the BAMBI antibody.
The isolation of leukocytes from murine kidneys was performed as described elsewhere (Kinsey et al., 2009) with slight modifications.
It was shown in these and in our studies that the uptake is mainly specific as a consequence of physiologically expressed PSMA in murine kidneys [18, 32].
Injection of MKPC rescued renal damage when administered to IRI damaged murine kidneys, and incorporation of MKPCs into some renal tubules was observed [114].
Only 9 sexually dimorphic transcripts were common to healthy human and murine kidneys and five showed differential regulation in both human and murine diseased kidneys.
The majority of genes showing gender biased expression either in diseased human and murine kidneys were different from those differentially expressed in healthy kidneys.
Our studies highlight significant differences in human and murine kidneys both at baseline and in their response to injury.
Murine kidneys contain both isoforms of 11β-HSD.
For RNA and DNA extraction, primary murine kidney fibroblasts were incubated with serum-free media containing 5′-Azacytidine (100 or 150 μg/ml in PBS, Sigma, St . Louis USA), Hydralazine (10 or 50 μg/ml in PBS, Sigma, St . Louis USA) or doxycycline (DOX, 1 μg/ml, Clontech, Mountain View, USA).
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