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Leptospiral LPS was shown to be recognized by both TLR2 and TLR4 in murine cells [14] whereas leptospiral lipoproteins were recognized by TLR2 in murine kidney epithelial cells [16].
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Here, we evaluated the toxicity of four model compounds in adherent cell cultures from three different species: human cervical (HeLa) and breast cancer cells (MCF-7), murine fibroblasts and kidney epithelial cells from African green monkey (Vero 76).
Murine macrophages (RAW 264.7, 23ScCr) and human kidney epithelial cells HEK293 were grown in Dulbecco's Eagle medium supplemented and incubated as mentioned above.
Murine macrophages (RAW 264.7 and 23ScCr) and human kidney epithelial cells HEK293 were grown in Dulbecco's Eagle medium supplemented and incubated as noted above.
Rabbit kidney epithelial (RK13) cells expressing full-length murine PrP (moRK13) were generated as described previously (Haigh et al., 2009).
moRK13 cells [rabbit kidney epithelial (RK13) cells expressing full-length murine PrP] readily propagate infectious prions and the line remains viable indefinitely, allowing the study of chronic cellular infection.
The cytotoxic potential of the lapachones evaluated was also assayed using non-tumor cells: human peripheral blood mononuclear cells, two murine fibroblast lines (L929 and V79 cells) and MDCK (canine kidney epithelial cells).
Murine macrophage cell line RAW264.7 (ATCC TIB-71) and human kidney epithelial cell line 293T (ATCC CRL-3216) were maintained in DMEM supplemented with 5% penicillin and streptomycin and 10% heat-inactivated fetal bovine serum (Gibcol).
MDCK kidney epithelial and MCF-7 breast carcinoma cell lines were stably transfected with constructs encoding full-length murine Podocalyxin and the morphologies of resulting bulk populations were examined by transmission electron microscopy (TEM).
Madin-Darby canine kidney epithelial cell.
Cytotoxicity was assessed in vitro on monkey kidney epithelial cells.
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