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We have therefore developed a new model of murine immunosuppression using both FK506 and hydrocortisone.
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There was increased mortality from pulmonary aspergillosis in a transplant-relevant immunosuppression model using both FK506 and hydrocortisone as compared with immunosuppression using hydrocortisone only.
Further studies are needed to search for indices of immunosuppression using more specific markers.
Neonatal murine skin was used to isolate murine SKPs as described by Toma et al. (2005).
To this end, we used a murine pancreatic cancer model using PAN02, a murine pancreatic adenocarcinoma.
From 1950s to 1970s syngeneic murine models were used.
Murine splenocytes were used freshly after preparation.
Primers for murine TLRs were used as reported previously [57].
Murine mAbs were used in initial clinical trials.
As immunosuppression we used the Edmonton protocol consisting of rapamycin plus FK506 plus anti IL-2Rα anti IL-2Rα[38].
No immunosuppression was used.
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