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In conclusion, we have developed a highly reproducible method to isolate endothelial cells from murine heart using cell surface markers Sca-1+, CD31+ and CD45−.
We explored the anti-arrhythmic efficacy of K+ channel activation in the hypokalaemic murine heart using NS1643 and nicorandil, compounds which augment IKr and IKATP respectively.
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Consequently, we set out to characterize the implications of the transient TRPC3 current for electrical and mechanical function of murine hearts using the isolated perfused Langendorff preparation.
We then proceeded to investigate the basis of these anti-arrhythmic effects of NS1643 and nicorandil in the hypokalaemic murine hearts using established arrhythmia provocation protocols.
The present study provides a first direct quantitative comparison of volume-based evaluation of in vivo imaging data and in vitro data on the uptake of SPECT tracers in murine brain and heart using [I]IBZM and [99mTc]sestamibi, respectively.
Secondly, we quantified the anti-arrhythmic efficacy of NS1643 and nicorandil in the hypokalaemic murine heart by using an established clinical method of arrhythmia provocation.
Secondly, we quantified the anti-arrhythmic efficacy of NS1643 and nicorandil in the hypokalaemic murine heart by using the established arrhythmia provocation protocol of PES.
Since mPR59/B δ1 and mPR59/B δ3 are putatively novel, and to further confirm their existence, we performed a reverse transcription on NIH 3T3 and murine heart RNA using primer E3 annealing in the mPR59 3' UTR.
To focus incident light on the intact open chest murine heart, we used a telecentric scan lens (SL, Thorlabs).
Murine hearts were used in view of their potential utility in future atrial studies of the effects of genetic modifications.
Total RNA from NIH 3T3 cells and murine heart tissue was isolated using GenElute™ Mammalian Total RNA Kit (Sigma) and reverse transcribed using primer E3 (5'-CTG TGC GCC CAG GAA CTG CGC-3') for the mPR59 isoforms and primers E5 (5'-CCT TGC AGT CCT CTT CCA CCA GC-3'), E7 (5'-GAT TCT GTC AGG GCT CTA GCA GG-3') and E9 (5'-GGA TGC TGT GTA GAG ATG CTC TAG-3') for the mPR72/130 isoforms.
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