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Thus, although short-term pacing in the wild-type murine heart is not associated with measurable electrophysiological effects, pacing in the setting of reduced baseline Cx43 expression results in significant prolongation of refractoriness and altered repolarization.
Genetically modified murine hearts are increasingly used as models of human cardiac disease (London, 2001).
The overall ratio in the atria of WT murine hearts was thus 1 : 420.
Murine hearts were excised from terminally anesthetized and heparinized C57B mice (body weight 20 25 g).
Murine hearts were embedded in Tissue-Tek O.C.T compound (Sakura Finetek) and prepared for sectioning and staining.
In a recent study on myocardial hibernation, murine hearts were analysed by a combined transcriptomic, proteomic, and metabolomic approach.
Murine hearts were washed to remove blood and aseptically isolated after incision at the base of the aorta.
Murine hearts were used in view of their potential utility in future atrial studies of the effects of genetic modifications.
Furthermore, ratios between the β3 and Nav1.5 mRNA expression levels in the LA and RA appendages of WT murine hearts were 1 : 470 and 1 : 380 respectively.
Alterations in ECG QT intervals correlate with the risk of potentially fatal arrhythmias, for which transgenic murine hearts are becoming increasingly useful experimental models.
For isolated heart measurements murine hearts were taken at 6 and 12 h post-CLP, and mounted with retrograde perfusion at 100 mmHg constant pressure with modified Krebs Henseleit buffer in an isolated heart apparatus (Hugo Sachs Elektronik), as previously described [ 13, 42– 42].
