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The alignment tool BLAT was used to map human probe sequences to the murine genome sequence database.
The oligonucleotide sequences and arrays were designed using as a reference the murine genome sequence from the Ensembl release 46.
With the availability of the complete murine genome sequence, of gene discovery approaches, and of genetic strategies to conditionally turn on or turn off potential target genes in the murine mammary gland, we anticipate that progesterone's mammogenic effects will be readily exposed to molecular dissection in the near future.
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Since the completion of the human and murine genome sequencing projects the location of the vector in the cellular genome can be defined precisely, allowing the determination of possible vector integration induced effects on the surrounding genomic DNA regions at the molecular level.
The target sequence for silencing the Tnfrsf1a gene [EMBL: M60468] was ATCTTCGGTCCTAGTAACT (base pairs 1095 to 1113), and we used ACTCATGTCTTGATCAGCT (no complementary sequence in murine genome) as scrambled control sequence.
Such hits may have been generated due to the currently incomplete annotation of the murine genome, or due to sequencing errors in the MPSS experiments.
By BLASTN analysis of the murine genome using the murine cDNA sequences, we could solve the genomic organisation of these three murine B" genes.
A BLAST search against the complete human and murine genome verified that the selected sequences were specific for the respective target gene.
We mapped the published UCE sequence to the unmasked murine genome and, through the use of bioperl scripts, we also retrieved the sequences 500 bp upstream and downstream of the mapped UCE and designed probes in these regions.
This indicated that murine hepatitis virus was a likely cause of the infection in transplant-recipient SCID mouse liver.> A total of 43 PCR products of varying sizes (312 bp-1757 bp) and a 3′-RACE product (1365 bp) were sequenced on both strands to derive the full-length genome sequence of the murine hepatitis virus.
A non-targeting pTER construct contained a similar sequence that had no homology to the human or murine genome, and was used as a negative control.
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