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Lymphoid cells stimulated by soluble tumour antigens in the MCA-induced murine fibrosarcoma system have been identified by subclass and protective capacity in adoptive syngeneic hosts.
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Compound (530 531) showed modest anti-proliferative activity against murine fibrosarcoma WEHI 164 cells (IC50 230 , 150µg/mL).
Three human sarcomas (leiomyosarcoma SK-LMS-1, liposarcoma SW872 and fibrosarcoma SW684) and one murine fibrosarcoma (KHT) were grown in nude and C3H/He mice, respectively.
Compound 330 showed most active anti-proliferative activity against bovine endothelial GM7373 (IC50, 48 µg/mL) and WEHI 164 murine fibrosarcoma cells (IC50 > 55 µg/mL) [175].
Murine fibrosarcoma TNF-sensitive L929s cells were cultured for 24 h by seeding 20,000 cells/well in 96-well plates, incubated at 37 °C with 5% CO2.
The IC50 values obtained after 24 h treatment of cell cultures with the extracts were 150 and 60 µg/mL on for murine fibrosarcoma cell line L929sA, known for its sensitivity to TNF, and MCF7, an ER positive control cell line, respectively.
Here the immunocompetent C57BL/6 mice model was treated with syngeneic murine fibrosarcoma cells (BFS-1).
Similarly, endogenous SIRT1-ΔExon8 protein also migrated at 300 kDa in murine fibrosarcoma cells (Figure S8).
The proliferating activity of fibroblasts and also murine fibrosarcoma cells first increased after treatment with low concentrations of the peptide.
The murine fibrosarcoma cell line BFS-1wt (generously provided by Prof. T. Hehlgans, Department of Immunology, Regensburg, Germany) were maintained in RPMI 1640 supplemented with 10% FCS and antibiotics.
Note that fibroblasts and also the murine fibrosarcoma cell line BFS-1 show an increase of proliferation after incubation with low concentration of the peptide.
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