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The IC50 values obtained after 24 h treatment of cell cultures with the extracts were 150 and 60 µg/mL on for murine fibrosarcoma cell line L929sA, known for its sensitivity to TNF, and MCF7, an ER positive control cell line, respectively.
Note that fibroblasts and also the murine fibrosarcoma cell line BFS-1 show an increase of proliferation after incubation with low concentration of the peptide.
In vitro the human synovial sarcoma cell line SW982, the murine fibrosarcoma cell line BFS-1 and primary human fibroblasts as a control were exposed to [D]-K3H3L9, a 15mer D,L-amino acid designer HDP.
The murine fibrosarcoma cell line BFS-1wt (generously provided by Prof. T. Hehlgans, Department of Immunology, Regensburg, Germany) were maintained in RPMI 1640 supplemented with 10% FCS and antibiotics.
The murine fibrosarcoma cell line L929 was purchased from Sigma-Aldrich (St . Louis MO, USA).
MC-GP is a murine fibrosarcoma cell line with high endogenous Cox-2 expression (Supplementary Figure 3C).
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Compound 330 showed most active anti-proliferative activity against bovine endothelial GM7373 (IC50, 48 µg/mL) and WEHI 164 murine fibrosarcoma cells (IC50 > 55 µg/mL) [175].
Here the immunocompetent C57BL/6 mice model was treated with syngeneic murine fibrosarcoma cells (BFS-1).
Similarly, endogenous SIRT1-ΔExon8 protein also migrated at 300 kDa in murine fibrosarcoma cells (Figure S8).
The proliferating activity of fibroblasts and also murine fibrosarcoma cells first increased after treatment with low concentrations of the peptide.
Necroptosis was first observed in L929 murine fibrosarcoma cells as a regulated form of cell death.
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