To further confirm this finding in in vivo murine experiment, we delivered adenovirus-LETM1 to K-ras LA1 mice via aerosol and examined the changes in protein expression of apoptosis-related proteins.
As well, in ABT-737/Hipp synergy experiments, we utilized engineered murine lymphomas where the manipulated anti-apoptotic family members had been introduced into mature tumors.
Since primary microglia are not available in quantities required for parabolic flight experiments, we used the murine cell line BV-2, whose function resembles that of tissue macrophages and which share many properties with both peripheral macrophages and monocytes [ 20].
For in vivo experiments, we used the subcutaneous murine Teflon chamber model developed in our laboratory and described earlier [16].
Using Q-PCR experiments, we examined undifferentiated and differentiated murine and human cultured podocytes to determine the expression levels of the actin depolymerizing factor/cofilin (AC) family.
In parallel experiments we analyzed the capacity of all murine and human synthetic CDRs to stimulate cytokine production by a non homogeneous cell population such as murine splenocytes.
For all experiments we used 6 week-old, female, murine pathogen free Swiss albino mice, belonging to the strain Udea ICR CD-1), and weighting 23–27 g [ 17].
Using a combination of iterative database searches and reverse transcriptase-polymerase chain reaction (RT-PCR) experiments we assembled contigs for cDNA encoding human, murine, puffer fish, and invertebrate TMC proteins.
In the present series of experiments, we evaluated whether delivery of a species-specific (murine) version of GC1 to cones of the postnatal GC1KO mouse could restore function to and preserve these cells.
We should add that in some earlier experiments we also measured the mobility of GFP-tagged murine PIPkin I α, and not surprisingly it showed parameters very similar to PIPkins Iγ90 and Iγ87 (D. Brough, M-L.G. and R.F.I., unpublished).
