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To further confirm this finding in in vivo murine experiment, we delivered adenovirus-LETM1 to K-ras LA1 mice via aerosol and examined the changes in protein expression of apoptosis-related proteins.
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In our murine experiments, we observed the induction of T cell anergy by a single injection of lowGM-DC into allogeneic mice ([23] and unpublished observations).
In fact, in a murine experiment, BBB disruption during the first 24 hours of hyponatremia was associated with a 70% risk of demyelination [ 12].
A very recent paper from David Fisher's group at Harvard described a murine experiment which suggested a deleterious effect of pheomelanin in terms of melanoma risk even in the absence of sunlight.
While murine experiments indicate that IL-2 is required for expansion of Treg, we have shown that IL-2 up-regulates expression of Bcl-2 and Foxp3 in human Treg and that Treg suppressor functions depend on the IL-2 concentration in their microenvironment [43].
All murine experiments were conducted in compliance with animal protocols approved by the Chinese Academy of Science Institutional Animal Care and Use Committee.
However, Lunding and colleagues showed in murine experiments that exacerbation of experimental asthma depends on IL-17A which is produced by NK and not Th17 cells [33].
Data from in vivo murine experiments were analyzed using a one-way ANOVA with treatment specified as the main effect.
The expression of foxp3 in human omentum was diminished in keeping with results in our murine experiments.
In this study, all in vivo murine experiments were performed on the C57BL/6 background, where in our hands, 80 100% of wildtype (WT) mice develop symptoms of ECM.
KB and KS conducted all murine experiments and data analysis.
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