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Wnt11 promotes cardiac differentiation via the non-canonical pathway in Xenopus [12] and murine embryonic cell lines [19].
C3H cells (C3H10T1/2), a murine embryonic cell line, rarely undergo spontaneous transformation, and are one of the few standard test systems for studying potential carcinogenic agents in animals or humans [ 5].
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TRIM28 restricts MLV LTR-driven transcription in murine embryonic cells [12].
To further study the roles of mitochondria in RIBE, in this study, murine embryonic cells lacking cytochrome c (Cyt c) were used as a genetic model.
Second, the LTR sequences of pMigR are of MSCV/MESV (murine stem cell virus/ murine embryonic stem cell virus) origin while those of pPRIG are of PCMV (PCC4-cell passaged myeloproliferative sarcoma virus [ 20]) origin.
Previously, we developed a scalable murine embryonic stem cell differentiation environment which maintained cell viability and supported ES cell differentiation to hepatocyte lineage cells.
We then depleted porcine oocytes of their mtDNA using 10 µM 2',3'-dideoxycytidine and transferred murine somatic cells along with murine embryonic stem cell extract, which expressed key pluripotent genes associated with reprogramming and contained mitochondria, into these oocytes.
We demonstrated this procedure using commercially available, mass-produced murine embryonic stem cell- and induced pluripotent stem cell-derived myocytes compared with a neonatal mouse ventricular myocyte target phenotype in coupled in vitro assays.
In this study, we describe a novel in vitro cell culture system for derivation of urothelial cells from murine embryonic stem cells (ESCs) following cultivation on collagen matrices in the presence all trans retinoic acid (RA).
Telomerase activity was high in the murine embryonic stem cell line CGR8.8, WRL 68 human embryo liver cells, testis, ovary and liver of adult mouse and rat.
Murine embryonic stem cell line D3 (wild type (wt) and E-cadherin-/ (Ecad-/) ES cells [19] were maintained in an undifferentiated state as described previously unless otherwise stated [17], [20].
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