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A murine dendritic cell line (DC2.4) co-expressing the hNIS and effluc genes (DC/NF) was established.
The DC2.4 cells, previously characterized as an immature murine dendritic cell line, were obtained from Dr. Kenneth L. Rock (Dana-Farber Cancer Institute, Boston, MA) [38].
The murine dendritic cell line, DC2.4, was cultured in RPMI1640 medium supplemented with 10% fetal bovine serum (Hyclone, Logan, UT), 0.05 mM β-mercaptoethanol (Sigma-Aldrich, St . Louis MO), 1% non-essential amino acids (Gibco, Grand Island, NY), and 1% penicillin-streptomycin (Gibco) at 37 °C in 5% CO2 atmosphere.
As source for the endolysosomal hydrolases we employed a commercially available murine dendritic cell (DC) line [21].
The murine dendritic cell GRNs were constructed using the interactions reported by Garber et al. (2012) available at http://www.weizmann.ac.il/immunology/AmitLab/data-and-method/iChIP/data.
Herein, we investigated the vaccine efficiency of DC2.4 cells, a murine dendritic cell line, pulsed with ovalbumin (OVA) in the murine E.G7-OVA tumour model after immunization via various routes.
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KP binds to murine dendritic cells and macrophages and up-regulate co-receptor expression, thus sustaining CD4+ lymphocyte activation.
Conversely, induction of autophagy with rapamycin inhibits the secretion of IL-1β in murine dendritic cells in response to LPS with ATP or alum.
Thus, N. caninum-induced activation of murine dendritic cells, but not that of macrophages, was associated with T cell IFN-γ production after IL-12 secretion.
Targeting of antigens to Fcγ receptors (FcγR) on human and murine dendritic cells (DC) has been shown to enhance DC maturation and both humoral and cellular immune responses.
The BAPCs-DNA nanoparticles enhanced the vaccine-induced antitumor protection and promoted activation of murine dendritic cells without significant toxic effects.
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