Sentence examples for murine cortactin using from inspiring English sources
Cttn 1 84 was made by amplification of the N-terminal 84 amino acids of murine cortactin using primers 5'-gagagaattcatgtggaaagcctctgc-3' (forward) and 5'-gagagtcgacatagccgtgggaagcctt-3' (reverse) and cloning into EGFP-MBD-C2.
To do this, we depleted cortactin using RNAi.
Cutoff percentages for dichotomization of the data were determined for cyclin D1 and cortactin using the median percentage of stained cells; 23% for nuclear cyclin D1 and 13% for cytoplasmic cortactin staining (Table 1).
Murine GFP-tagged expression constructs were produced using pcDNA3FLAG2AB wild-type murine cortactin [12] as the template for mutagenesis, then subcloned as EcoRI-KpnI PCR fragments into pAcGFP-C1 (Clontech, Mountain View, CA).
For generation of the latter, murine cortactin (genebank accession: NM_007803) was cloned into pEGFP-C1 vector.
By BLASTN analysis of the murine genome using the murine cDNA sequences, we could solve the genomic organisation of these three murine B" genes.
Importantly, forced expression in myotubes of a tyrosine phosphorylation-defective cortactin mutant (but not wild-type cortactin) suppressed agrin-dependent AChR clustering, as did the reduction of endogenous cortactin levels using RNA interference, and introduction of the mutant cortactin into muscle cells potently inhibited synaptic AChR aggregation in response to innervation.
Forced expression of the same 3YF-mutant cortactin in myotubes, or the suppression of cortactin expression using RNAi, inhibited agrin-induced AChR clustering in this study.
The HA-induced phosphorylation of paxillin was attenuated by depletion of CD44 and cortactin expression using selective RNAi strategies, suggesting that it is a downstream target of HA-CD44-cortactin signaling.
Neonatal murine skin was used to isolate murine SKPs as described by Toma et al. (2005).
Importantly, a phospho-mutant (3YF) cortactin was used to demonstrate that src-dependent enhancement of cortactin's ability to stimulate Arp2/3 (through Nck1 and N-WASP or WIP) requires the Y421, Y466 and Y482 sites of cortactin [33].
