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CpG-ODN 1668 (for murine cells) was purchased from Sigma-Aldrich.
Reconstitution from transplanted murine cells was further supported by the use of GFP-tagged ES cell line.
The organization of actin filaments in EHV-1-infected and control neuronal murine cells was examined by confocal microscopy at 24, 48 and 72 h p.i.
Immediately after death, a thorough peritoneal lavage with PBS was performed, the mixed cell suspension obtained by this procedure was stored on ice and the total cell number (containing human myeloma and murine cells) was determined.
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To analyze BST-2 transcript levels, all human samples were normalized to HMLE cells and murine cells were normalized to E0771 shControl (shCTL) cells.
Moreover, the 4T1 murine cells are also typical triple negative breast cancer cell line and closely mimic human breast cancer considering characteristics as anatomical site, immunogenicity and growth development.
Adipocyte differentiation in murine cells is regulated mainly by CCAAT-enhancer-binding protein (C/EBP) α, β and δ and peroxisome proliferator-activated receptor (PPAR) γ, which induce the expression of genes that lead to the development of the adipocyte phenotype which includes the formation of lipid droplets and adipokine release (reviewed in Tang and Lane15).
The TC1 murine cells were grown in 96-well plates with 104 cells per well.
The morphological changes in TC1 murine cells were observed after 48 h with EB and EL extracts of A.officinalis.
To explore the functional importance of SMC1 phosphorylation, murine cells were generated, in which the two damage-induced phosphorylation sites in SMC1 are mutated.
Genome-wide studies of EIAV integration targeting in murine cells are presented in this section and analysis of HIV integration in murine cells is described in the next section.
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