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We showed a decrease in viral RNA levels in cell-free virus particles and a reduction reverse transcribed complementary DNA (cDNA) in virus-infected human and primary murine cells by incubation with ODNs.
HIV-1 infection was facilitated in murine cells by circumventing necessary HIV-1 co-receptors by using a HIV-1/VSV pseudotyped virus.
Furthermore, we have previously described that C7L insertion into the parental NYVAC genome restores the viral replication cycle in cultured human and murine cells by preventing eIF2-α phosphorylation and the induction of apoptosis [19].
However, we observed that closely related hESC-based systems showed very different responses to VPA (Krug et al. 2013) and apparent HDACi consensus genes (Jergil et al. 2009, 2011) defined in murine cells by overlapping responses of different drugs were not regulated in our cells.
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Both isolated subpopulations were essentially free of murine cells as detected by the absence of murine CD29 and CD45 expression (Figure S1B, Materials and Methods S1).
The organization of actin filaments in EHV-1-infected and control neuronal murine cells was examined by confocal microscopy at 24, 48 and 72 h p.i.
Figure 1 shows that at least a rough visual semi-quantitative evaluation of dilution mixtures of human in murine cells is possible by conventional DNA-PCR.
The malignant murine cells had arisen by transformation of murine stromal cells during the first subcultures in vitro, possibly caused by a factor produced by the human melanoma cells.
Reconstitution from transplanted murine cells was further supported by the use of GFP-tagged ES cell line.
On the other hand, different level of expression of antioxidant proteins such as Bcl-2 in coral host cells can lead to differential level of protection against oxidative stress induced by elevated temperature as it has been described in human and murine cell lines by Setroikromo et al [40].
Migration profiles of cTA12 light and heavy chains produced by either insect cells or by myeloma murine cells do not present any differences (Figure 2).
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