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Total RNA was isolated from murine brains using the GenElute™ Mammalian Total RNA Miniprep Kit (Sigma) according to manufacturer's instructions.
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There are only a few reports on measurements of [I]IBZM in the murine brain using SPECT or multi-pinhole SPECT, respectively.
Similar levels of PrPC from RK13MoPrP cells and murine brain were used in PMCA (Fig. 1A).
The present study provides a first direct quantitative comparison of volume-based evaluation of in vivo imaging data and in vitro data on the uptake of SPECT tracers in murine brain and heart using [I]IBZM and [99mTc]sestamibi, respectively.
However, our recent results obtained from murine brain distribution studies using direct brain perfusion show that P-gp restricts the accumulation of [H]cortisol in specific brain regions, such as hypothalamus, and does not restrict the access of [H]corticosterone in any brain regions sampled (13).
First, we acquired the emission spectra for DAPI-DNA using murine brain cells as a DAPI-DNA baseline that did not contain appreciable levels of polyPs.
Whilst other groups have examined biopsies from specific brain areas in humans [ 22] and by dissection in hamsters [ 18], we require a method for murine brains which is reproducible and can be used in a range of biochemical tests.
Murine brains were removed and frozen in solid CO2, sectioned using a cryostat, mounted on slides and allowed to air dry.
The method described is for the microdissection of murine brains.
We used a murine brain endothelial cell line bEnd.3 (bEnd.3, American Type Culture Collection, Manassas, Virginia, USA) for coculture experiments as described previously [15].
To compare our hypothesis of extravascular migration by melanoma with the migration of glioma cells, we have used the B16 murine melanoma cell line and the GL26 murine glioma cell line in an in vivo murine brain tumor model and in vitro using endothelial cells that have formed capillary-like structures and have been cocultivated with tumor cells.
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