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There are only a few reports on measurements of [I]IBZM in the murine brain using SPECT or multi-pinhole SPECT, respectively.
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Total RNA was isolated from murine brains using the GenElute™ Mammalian Total RNA Miniprep Kit (Sigma) according to manufacturer's instructions.
Similar levels of PrPC from RK13MoPrP cells and murine brain were used in PMCA (Fig. 1A).
The present study provides a first direct quantitative comparison of volume-based evaluation of in vivo imaging data and in vitro data on the uptake of SPECT tracers in murine brain and heart using [I]IBZM and [99mTc]sestamibi, respectively.
However, our recent results obtained from murine brain distribution studies using direct brain perfusion show that P-gp restricts the accumulation of [H]cortisol in specific brain regions, such as hypothalamus, and does not restrict the access of [H]corticosterone in any brain regions sampled (13).
First, we acquired the emission spectra for DAPI-DNA using murine brain cells as a DAPI-DNA baseline that did not contain appreciable levels of polyPs.
We used a murine brain endothelial cell line bEnd.3 (bEnd.3, American Type Culture Collection, Manassas, Virginia, USA) for coculture experiments as described previously [15].
We therefore analysed the developing murine brain and SNS for Gata-4 expression using immunohistochemistry.
RNA isolated from murine brain endothelial cell line (bEnd.3) was used a positive control.
In 1972, formic acid hydrolysis followed by chromatographic analysis was used to detect 5-hmC in murine brain and liver DNA.
To address this issue, we used whole-cell patch clamp recording in acute murine brain slices at both room and physiological temperatures to examine the effects of PKC-mediated phosphorylation on tonic inhibition.
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