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We showed a remarkable Cr depletion in the murine brain tissues and cognitive defects, thus resembling the key features of human CCDS1.
This feature is linked to a high ABC transporter activity, among which the ABCC6 transporter seems to be of importance, as was shown by its high mRNA levels in the murine brain tissues.
Genomic DNA was extracted from murine brain tissues (#200600, DNA Extraction Kit, Agilent Technologies, Inc. Santa Clara, CA 95051, USA) and from liver, spleen and gonads (#13343 Blood and Cell Culture DNA Midi Kit, Qiagen, Venlo, 5911 KJ, Netherlands).
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We show that UCP4 is present in fetal murine brain tissue as early as embryonic days 12 14 E12 E144), which coincides with the beginning of neuronal differentiation.
To further validate the physiologic significance of this, we used silver-enhanced immunogold immunohistochemistry of thin sections of murine brain tissue to demonstrate that there are RyR present in neuronal mitochondria visualized as distinct punctae that co-localized exclusively with the inner mitochondrial membrane (IMM) (Figure 5A).
Moreover, individual mitochondria may display different bioenergetic-specific requirements for specific CLs as described for murine brain tissue.
Within the murine brain, tissue analysis results originate from three discrete areas in relation to the tumor-glioma, peritumoral area, and the contralateral tumor free hemisphere.
To establish the spatial resolution limits of our photo-tagging procedure, we tagged single-voxel spots within sections of methanol-fixed murine brain tissue.
As shown in Figure 2, the murine brain tissue constitutively expressed low levels of NOD1 and NOD2 proteins or failed to express these proteins at all.
Finally, during the preparation of this manuscript, Toghi Eshghi et al. and Powers et al. demonstrated the application of N-glycan MALDI imaging to FFPE murine brain tissue [ 17], murine kidney, gliomas, and tissue microarrays [ 18].
To determine whether the murine brain tissue expressed these novel pattern recognition receptors, we assessed the NOD1 and NOD2 mRNA levels in the uninfected and S. pneumoniae-infected murine brains.
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