Sentence examples for murine brain sections from inspiring English sources

To minimize variations in experimental conditions, both human and murine brain sections of all specified ages were incubated with the same solution of antibodies and treated altogether in one experimental session.

In situ hybridization on murine brain sections was employed to determine sites of altered Txnip expression.

Using in situ hybridization analysis, we were also able to detect a reproducible global increase in C1qA expression in murine brain sections that was apparent by 16 months of age.

41 Our immunostainings of murine brain sections revealed that neuroserpin is expressed by perivascular SST-positive cells (Fig. 4A), whereas tPA is expressed by VIP-positive cells (Fig. 4B).

The DAPI-DNA emissions for Figure 10A were collected from a murine brain section, while Figure 10B depicts the emission spectra from a solution of 10 µg/mL sodium polyphosphate (Type 28, Sigma-Aldrich) and 10 µg/mL DAPI in TRIS (0.2 M, pH 9).

To investigate the potential association of neuroserpin and tPA with cerebral vessels we performed immunofluorescent staining on WT murine brain tissue sections and analyzed the expression of neuroserpin and tPA by confocal microscopy.

45 Immunofluorescent staining of WT murine brain tissue sections confirmed expression of PDGFR α in GFAP-positive perivascular astrocytes in the neurovascular unit (Fig. 6A) and showed that the receptor is distributed in the border between the astrocytes and the vascular mural cells, here visualized by ASMA (Fig. 6B).

Moreover, an analysis of murine CCM3-KO brain sections showed p62 clusters in the surrounding area of vascular malformations (Fig 3G).

To further validate the physiologic significance of this, we used silver-enhanced immunogold immunohistochemistry of thin sections of murine brain tissue to demonstrate that there are RyR present in neuronal mitochondria visualized as distinct punctae that co-localized exclusively with the inner mitochondrial membrane (IMM) (Figure 5A).

To establish the spatial resolution limits of our photo-tagging procedure, we tagged single-voxel spots within sections of methanol-fixed murine brain tissue.

In contrast, staining for endogenous murine SOD1 in PET-blots of wild type (WT) mice brain sections which appears relatively uniformly distributed over the blot with predominantly cytoplasmic staining as shown for the medulla region does not resist proteolytic digestion with 5 μg/ml protease K for 15 h.