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Binding to glioma cells, co-delivery of siRNA and DTX in human glioblastoma cells (U87 MG) and murine brain microvascular endothelial cells (BMVEC), VEGF gene silencing, antiproliferation and anti-tumor effects of the dual peptides-modified liposomes were evaluated in vitro and in vivo.
Control (i.e., pMSCV-IRES-GFP) and LCN7 retroviral supernatants were produced and infected into murine brain microvascular MB114 endothelial cells and SVEC tumor derived endothelial cells as described previously [36], [37], [38], [39].
The expression of CD105 in brain after stroke has not been studied, however, CD105 expression was induced by hypoxia in murine brain microvascular EC via mitogen activated protein kinase (MAPK) pathways [ 12] suggesting it is also a marker of active neovessel formation in ischaemic tissues.
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