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RVG29 functionalized liposomes (RVG29-lip) showed significantly higher uptake efficiency in murine brain endothelial cells and dopaminergic cells, and high penetration efficiency across the blood brain barrier (BBB) in vitro.
We found that AAV-GMN, but not AAV2, readily transduces the murine brain endothelial cell line bEnd.3, a result that mirrors previously observed in vivo transduction profiles of brain vasculature.
RNA isolated from murine brain endothelial cell line (bEnd.3) was used a positive control.
We used a murine brain endothelial cell line bEnd.3 (bEnd.3, American Type Culture Collection, Manassas, Virginia, USA) for coculture experiments as described previously [15].
We used a recently described murine coculture model consistent of cortical organotypic slice cultures (COSC) and the murine brain endothelial cell line bEnd.3 as documented in detail before [15].
Claudin-3 expression in primary human, rat and murine brain endothelial TJs is no longer of debate since antibodies that are not cross-reactive to claudin-1 have been generated [ 5- 7].
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In vitro transfection of murine brain capillary endothelial bEnd.3 cells with pSPE, pTETA, and pLPEI polyplexes was 2.3 4.9 times more effective compared with the non-degradable LPEI 22 kDa reagent (ExGen500) in the presence of serum.
Binding to glioma cells, co-delivery of siRNA and DTX in human glioblastoma cells (U87 MG) and murine brain microvascular endothelial cells (BMVEC), VEGF gene silencing, antiproliferation and anti-tumor effects of the dual peptides-modified liposomes were evaluated in vitro and in vivo.
Al (14 C-citrate uptake into murine-derived brain endothelial cells appeared to be carrier mediated, Na independent, pH independent, and energy dependent.
The synthetic cannabinoid WIN 55,212-2 increases COX-2 expression and PGE(2) release in murine brain-derived endothelial cells following Theiler's virus infection.
Control (i.e., pMSCV-IRES-GFP) and LCN7 retroviral supernatants were produced and infected into murine brain microvascular MB114 endothelial cells and SVEC tumor derived endothelial cells as described previously [36], [37], [38], [39].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com