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In order to test this hypothesis, we generated both wild-type (WT) and PPARγ-null trophoblast stem (TS) cells, from E3.5 murine blastocysts using previously published methods [14].
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Genomic DNA was isolated from blastocysts using the Qiagen DNA Micro kit (Qiagen).
The IVF blastocysts used here were all expressed Y-linked genes (ZRSR2Y, USP9Y, UTY, DDX3Y, PRAME, and EIF2S3Y), whereas SCNT blastocysts were female.
The blastocysts used in the study are shown in Supplemental Figure 1 (available online).
To determine whether BNP, NPR-A and NPR-B are expressed in pre-implantation embryos, 3.5-day-old murine blastocysts were subjected to double-immunofluorescence analysis using anti-BNP, anti-NPR-A, or anti-NPR-B antibodies, as well as an antibody against Oct-4 (self-renewal marker).
Zhang, J. et al. Systematic characterization of the murine mitochondrial proteome using functionally validated cardiac mitochondria.
Day 7 expanded blastocysts and hatched blastocysts were used to derive the ESC lines.
rES cells were injected into the blastocyst cavity using a microinjection pipette.
In other studies the blastocyst rate using PFFs ranged from 11.9 to 31.2% [ 12, 16, 33, 34], which is several times higher compared to our blastocyst rate.
RNA extracted from the ICM was compared with total blastocyst RNA using genome-wide expression analysis.
To this end, we used a murine pancreatic cancer model using PAN02, a murine pancreatic adenocarcinoma.
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