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Nearly all of the bivalent transcription factors in QSCs are PcG targets in murine ESCs, including many Hox genes (Boyer et al., 2006).
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Examples of hybrid ESCs include, but are not limited to, purely continuous ESC with optimizers described by set-valued mappings, ESC with arbitrarily fast and slow switching modes, ESC with weakly-jumping parameters, as well as distributed ESCs for multi-agent systems with time-varying graphs.
Hurdles to adopting ESCs include teratoma formation and host immunorejection [ 17, 18].
These results coupled with observations from this study may suggest the existence of a signaling network in murine ESCs wherein selective RAR nuclear receptor complexes including RARγ and RARβ2 induce GATA4 and GATA6 expression leading to UP upregulation.
Here, we test the hypothesis that tissue-specific ECM influences the differentiation of murine ESCs.
Murine ESCs or iPS cells were differentiated 11 d in vitro and cocultured 5-7 d with irreversibly injured myocardial tissue slices.
Treatment of murine ESCs with insulin or insulin-like growth factors (IGF1/2) during early differentiation increased mesodermal cell proliferation and, consequently, CPC formation.
Recently, we showed that suspension bioreactors could be used in the regulated large-scale expansion of highly pluripotent murine ESCs.
Maintenance of culture pH was achieved via a perfused rotary bioreactor while murine ESCs were encapsulated in alginate hydrogels, which served as a three-dimensional (3D) platform and matrix support for the ESC culture.
In this study, we demonstrated how sensitive ESCs are to pH, utilizing murine ESCs as a cell model and experiments conducted at three different pH conditions (pH 6.8, 7.1 and 7.4).
In this study, we developed an efficient culture strategy to induce the differentiation of murine ESCs (mESCs) into dental epithelial cells.
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