Therefore, the authors also used the murine DC cell line JAWS II.
Antigen presentation by murine DC cells can be inhibited by S. Typhimurium [ 34, 35], and we observed that both CD80 and CD86 were down-regulated at 8 hpi in S. Typhimurium infected MLN [ 12].
By comparing two different inhibitors of the Rsk (p90 ribosomal S6 kinase) kinases, we found that the increasingly used compound BI-D1870 had biological effects in murine DCs (dendritic cells) that could not be solely ascribed to Rsk or other documented targets.
We here established a possible breakthrough, a simple and cytokine-based culture method to realize a log-scale order of functional murine DCs (>1,000-fold >1,000-foldls whichused as a model before moving to human studies.
GCs decrease the co-stimulatory molecule expression in murine DCs, and therefore these cells have a poor T-cell stimulatory capacity in vitro and in vivo (Moser et al, 1995).
In our studies, we showed that OVA protein could be presented by murine DCs to CD8 positive T cells efficiently with the help of γFe2O3/APTS.
Recombinant adenovirus technology has been widely employed in gene therapy and for delivering genes into primary cell types including murine DCs (Ribas et al, 1997; Song et al, 1997; Wan et al, 1997).
These results support our previous finding of CPMV particle localization within LAMP-2 and ß-COP-bearing Golgi and lysosome organelles both in BalbCl7 cells [24] and murine DCs [25].
In the present study, we used replication deficient adenoviruses to introduce a tumor-associated Ag (a truncated human prostate-specific membrane antigen (tPSMA)) and the T cell costimulatory molecule 4-1BBL into murine DCs, and observed the ability of these recombinant DCs to elicit tPSMA-directed T-cell responses in vitro and anti-tumor immunity to RM-1-tPSMA in a murine tumor model.
Salmonella infection of murine DCs has been reported to inhibit presentation of antigen to CD4+ T cells [ 14, 28].
