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Plymouth routines in multivariate ecological research version six.
Plant species diversity was assessed using Plymouth Routines in Multivariate Ecological Research version six (PRIMER 6) to conduct non-parametric multidimensional scaling (nMDS) and hierarchical cluster analysis of plant composition at Oaky Creek, Blackwater, and Moura mine sites.
All multivariate analyses were performed using PRIMER (Plymouth Routines In Multivariate Ecological Research) v5® software.
These data were analyzed using the PRIMER software package (Plymouth Routines in Multivariate Ecological Research, version 6) [38].
As species richness and species extinction rates depend on abundance, we used the only abundance data available, estimated to the nearest power of two (1, 2, 4, 8, 16, etc)., to calculate rarified species richness using Primer (Plymouth Routines in Multivariate Ecological Research, v.6).
Ordinations, ANOSIM and SIMPER routines were performed using the PRIMER 6 package (Plymouth Routines in Multivariate Ecological Research 2010).
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